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Advanced
Bio-coating Technologies
Biokeystone is
the expert of advanced bio-coating technologies. Based on expertise of
leading scientists, engineers, and state of the art facilities,
Biokeystone has established as the leader of coated German glass
coverslips for a variety of challenged primary cell culture and primary
neuron cultures. You will find full line supplies of sterile German
glass coverslips in different sizes and thickness being professional
coated with poly-L-lysine, poly-D-lysine, collagen, fibronectin,
laminin, or gelatin.
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The
coating expertise Click
to enlarge
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This is a featured project of year 2010 in
collaborating with The Saban Research Institute, CHLA, University
of Southern California. The entire surface of a German
coverslip was coated with poly-L-lysine. By utilizing advanced
expertise and state of the art facilities at micrometer
resolution, scientists at Biokeystone can manipulate PLL in
such a way that some PLL molecules gain functions as guidance
cue (light green tracks) while other PLL molecules function as
merely attachment factor (dark green area). |
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MCI:
Microfluid Neuronal Culture
How
can our Microfluid Culture Insert make your neuron culture robust?
The
Problem:
Serious problems occur in most traditional
cell culture systems. For instance, conditioned culture medium
is essential to many cell types for optimal growth and
responsiveness, but typical large volumes of culture medium
dilute the concentrations of secreted cell factors and thus
significantly slow down and de-potentiate the life events of the
cells in culture. In contrast with in vivo environments, free
bulk flow of culture liquid perturbs local chemical gradients,
which may impair signaling and disturb cell behaviors. Resulting
assay data may be weak, excessively variable and potentially
misleading.
The
Solution:
Using
micro-fluidic design and engineering strategies, Biokeystone has
developed MCI, a new model for cell culture technology. With
MCI, the free flow of culture medium can be substantially
eliminated, stabilizing local gradient profiles of factors
secreted by cultured cells. At the same time, the volume of
immobilized culture medium surrounding the cultured cells is
reduced several hundred fold, which enables rapid accumulation
of secreted factors to high levels, accelerating cell
establishment, growth, differentiation, or maturation
accordingly. If needed, cells can be cultured successfully at
very low density. This innovative culture system more nearly
mimics in vivo conditions for effective cell signaling and
responsiveness, unlike what one sees in traditional
culture systems.
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6Mgel:
Run high-speed gel in dd-Water
As
shown below, 1x running buffer, a highly conductive liquid, has
two functions in conventional submarine agarose gel
electrophoresis: keeping the agarose gel cooled and conducting
electric current. To avoid gel overheating, the volume of
running buffer should be as large as possible. But more buffer
conducts more electric current, which generates more heat. So
buffer volume should be as small as possible. Which is correct - more buffer or less buffer?
The
principle of the 6Mgel invention
The
inventor recognized that deionized water is the best and
cheapest coolant. The electrical resistance of lab dd-water is
18MΩ,
which means that dd-water is an excellent electrical
insulator. By simply adding dd-water directly on top of a TBE
agarose gel, the desired effects are achieved: gels can be run
much faster! You can add a large volume of dd-water to
dissipate heat so that a much higher voltage can be applied to
drive faster migration of DNA molecules. Band
"smiling" is eliminated because the large volume of
dd-water in direct contact with the gel maintains even heat
distribution. Also, sharper banding occurs due to "stacking
effects of dd-water in gel wells.
In
6Mgel, the V/cm in wells is elevated about 3-fold higher than in
the gel body, which drives DNA 3 times faster in wells than in
the gel body, resulting in sharper bands. Furthermore, there is
no running buffer consumption.
The
strength of 6Mgel is particularly significant for Genotyping and PCR
applications, where high percentage agarose gels are usually required.
It takes longer time for sample molecules to migrate through the
concentrated gel body. 6Mgel innovation has solved the problem.
During
the development of 6Mgel, more and more features were added, such as
buffer-less high speed electrophoresis, lab-made precast gels for
high-speed electrophoresis, and agarose-saving gel systems. |
