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Microfluid
Cell Culture & Co-Cultures
Novel
Protocols to energize cell cultures easily
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MCI: Microfluid Cell Culture Insert
Generating
robust cell cultures and co-cultures easily. Especially powerful for
primary cell cultures and co-cultures. PLL/collagen coated, optically
clear,
sterile, ready to use...
MCI
is designed for all kinds of cell cultures. Significant improvement can
be expected if you have experienced difficulties culturing primary cells, such as primary neurons, primary wt pancreatic
cells, islet cells, or cancer stromal cells.
Product
format:
It
keeps your working habit of using multi-well plates for
cell cultures. Each unit of MCI can be placed into, and
detached from, wells of
multi-well plates.
You will still
perform
desired procedures the same way as usual, such as viewing and
imaging live neurons, changing culture medium, performing neuron transfections,
trypsinizing and
harvesting cells, fixing neurons and IHC,
or mounting cultured neurons on slides after staining.
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Pure live primary neurons in
culture viewed under phase contrast microscope. Pure
primary neuron cultured in defined simple culture medium.
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MCI:
Neuron Microfluid Culture Insert, US and international patents
pending, Micro structure and PLL coated, sterile, ready for
culture. |
Principle:
Why
MCI can energize your cultures?
The
Problems:
Serious problems occur in most traditional cell culture
systems. For instance, conditioned culture medium is essential to many
cell types for optimal growth and responsiveness, but typical large
volumes of culture medium dilute the concentrations of secreted cell
factors and thus significantly slow down and de-potentiate the life
events of the cells in culture. In contrast with in vivo environments,
free bulk flow of culture liquid perturbs local chemical gradients,
which may impair signaling and disturb cell behaviors. Resulting assay
data may be weak, excessively variable and potentially misleading.
The
Solution:
Using
micro-fluidic design and engineering strategies, Biokeystone has developed
MCI, a new model for cell culture technology. With MCI, the free flow of
culture medium can be substantially eliminated, stabilizing local
gradient profiles of factors secreted by cultured cells. At the same
time, the volume of immobilized culture medium surrounding the cultured
cells is reduced several hundred fold, which enables rapid accumulation
of secreted factors to high levels, accelerating cell establishment,
growth, differentiation, or maturation accordingly. If needed, cells can
be cultured successfully at very low density. This innovative cell
culturing
system more nearly mimics in vivo conditions for effective cell
signaling and responsiveness, unlike what one sees in traditional
culture systems.
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Biokeystone
The
Bio-Coating Expert
Coated
coverslips
for
neuron cultures
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Primary cell
and neuron
culture tested, starting $12/box
Primary cell
and neuron
culture tested, starting $36/box
Advanced
treatment of German glass coverslips, sterile, ready to coat your own
substance, starting $78/box
Professional
bio-coating technology, primary cell
culture tested, sterile, ready to use for cell and neuron cultures,
starting $89.50/box
Professional
bio-coating technology, primary cell
culture tested, sterile, ready to use for cell and neuron cultures,
starting $99.50/box
Professional
bio-coating with rat tail type I collagen, primary cell
culture tested, sterile, ready to use for cell and neuron cultures,
starting $109.50/box
Professional
bio-coating technology, primary cell
culture tested, sterile, ready to use for cell and neuron cultures,
starting $99.50/box
No
spin no purification
 Liberty
120 high speed gel
Features:
Little
buffer or buffer-less,
High-speed
electrophoresis in 6 minutes,
Agarose
saving,
Straight
banding technology,
One
tray for 3 gel
lengths, 48, 80, or 128mm
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