Principle of rapid gel electrophoresis

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electrophoresis principle

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6Mgel Principle

Rapid water cooling electrophoresis

US Patent 5,549,806

 

 

6Mgel - Rapid electrophoresis in 6 minutes

 

1x running buffer, a highly conductive liquid, has two functions in conventional submarine agarose gel electrophoresis: keeping the agarose gel cooled and conducting electric current.

horizontal gel electrophoresis diagram

To avoid gel overheating, the volume of running buffer should be as large as possible. But more buffer conducts more electric current, which generates more heat. So buffer volume should be as small as possible. Which is correct - more buffer or less buffer?

 

The principle of the 6Mgel invention

The inventor of 6mgel recognized that water is the best and cheapest coolant. The electrical resistance of lab dd-water is 18MΩ, which means that dd-water is an excellent electrical insulator. By simply adding dd-water directly on top of the buffer-containing agarose gel, the desired effects are achieved: gels can be run much faster!  You can add a large volume of dd-water to dissipate heat so that a much higher voltage can be applied to drive faster migration of DNA molecules. Band "smiling" is eliminated because the large volume of dd-water in direct contact with the gel maintains even heat distribution. Also, sharper banding occurs due to "stacking effects of dd-water in gel wells.

In 6Mgel, the V/cm in wells is elevated about 3-fold higher than in the gel body, which drives DNA 3 times faster in wells than in the gel body, resulting in sharper bands. Furthermore, consumption of running buffer can be reduced by 50-75%.

During the development of 6Mgel, more and more features were added, such as buffer-less high speed electrophoresis, lab-made precast gels for high-speed electrophoresis, and agarose-saving gel systems. 

   The Breakthrough of high-speed electrophoresis without running buffer

  See User Feedback about the performance of buffer-less high speed electrophoresis:

" The Liberty bufferless gel system is the best gel electrophoresis system we've ever used. It's not only easy to set up, robust, and fast, but generates excellent results without the need of running buffer. Highly recommended. See our genotyping service picture below." ---- Ivan Delgado Orlic, President and CEO, Mouse Genotype Co. 

 

 

6Mgel Innovative Tips

 

How to see PCR results in only 6 minutes?

How to run high-speed gel with no running buffer

Hot to eliminate agarose gel band smiling?

How to save agarose?

One-step DNA extraction for genotyping PCR

 

 

Liberty 120 high- speed gel system

One gel tray for 3 gel lengths

 

 

See Genotyping / PCR results in 6 minutes

 

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