The Expert of Primary Cell Cultures

Biokeystone      

Primary cell culture expert

Get protocols and tools of primary cell culture

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Primary Cell Culture

Generating accelerated primary cell culture easily

 US and international patents pending

 

MCI -- Microfluid Primary Cell Culture

Generating robust accelerated primary cell culture easily. Especially powerful for challenged primary cultures. Polylysine coated, optically clear, sterilized by radiation, ready to use. MCI is designed for challenged primary cell cultures. Significant improvement can be expected if you have experienced difficulties in your primary cell cultures.

The handling of MCI primary cell culture system is very simple:

It keeps your working habit of using multi-well plates for cell cultures. Each unit of MCI can be placed into, and detached from, wells of multi-well plates.

You will still perform desired procedures the same way as usual, such as viewing and imaging live neurons, changing culture medium, performing neuron transfections, trypsinizing and harvesting cells, fixing neurons and IHC, or mounting cultured neurons on slides after staining.

Cultured primary neuron in microfluid neuron culture system at low density, showing energized accelerated culture results, using defined simple culture medium with no FBS no glial cells. Click to enlarge the cultured neuron

MCI: Neuron culture Insert, US and international patents pending, Micro-structure and PLL coating made in USA, sterile, ready for primary neuron cultures

 

Cat #

Product Description 

Unit price

MCI-60-PLL

MCI microfluid primary cell culture insert, the simplest way to establish robust primary cell culture, specially effective for difficult primary neurons. Micro-structure and poly-L-lysine coating are made in California. German coverglass is manufactured by German coverslip manufacturers. 60 unit package,15 units/pack x 4 packs, pre-coated, pre-sterilized by radiation, ready to use, user manual included

$148.80

MCI-120-PLL

MCI microfluid primary cell culture insert, the simplest way to establish robust primary cell culture, specially effective for difficult primary neurons. Micro-structure and poly-L-lysine coating are made in California. German coverglass is manufactured by German coverslip manufacturers.120 unit package,15 units/pack x 8 packs, ready to use, pre-coated, pre-sterilized by radiation, user manual included

$248.80

MCI-240-PLL

MCI microfluid primary cell culture insert, the simplest way to establish robust primary cell culture, specially effective for difficult primary neurons. Micro-structure and poly-L-lysine coating are made in California. German coverglass is manufactured by German coverslip manufacturers.240 unit package,15 units/pack x 16 packs, ready to use, pre-coated, pre-sterilized by radiation, user manual included

$398.80

MCI-60-PDL

MCI microfluid primary cell culture insert, the simplest way to establish robust primary cell culture, specially effective for difficult primary neurons. Micro-structure and poly-D-lysine coating are made in California. German coverglass is manufactured by German coverslip manufacturers. 60 unit package,15 units/pack x 4 packs, pre-coated, pre-sterilized by radiation, ready to use, user manual included

$148.80

MCI-120-PDL

MCI microfluid primary cell culture insert, the simplest way to establish robust primary cell culture, specially effective for difficult primary neurons. Micro-structure and poly-D-lysine coating are made in California. German coverglass is manufactured by German coverslip manufacturers.120 unit package,15 units/pack x 8 packs, ready to use, pre-coated, pre-sterilized by radiation, user manual included

$248.80

MCI-240-PDL

MCI microfluid primary cell culture insert, the simplest way to establish robust primary cell culture, specially effective for difficult primary neurons. Micro-structure and poly-D-lysine coating are made in California. German coverglass is manufactured by German coverslip manufacturers.240 unit package,15 units/pack x 16 packs, ready to use, pre-coated, pre-sterilized by radiation, user manual included

$398.80

MCI-60-Collagen

MCI microfluid primary cell culture insert, the simplest way to establish robust primary cell culture, specially effective for difficult primary neurons. Micro-structure and collagen coating are made in California. German coverglass is manufactured by German coverslip manufacturers. 60 unit package,15 units/pack x 4 packs, pre-coated, pre-sterilized by radiation, ready to use, user manual included

$168.80

MCI-120-Collagen

MCI microfluid primary cell culture insert, the simplest way to establish robust primary cell culture, specially effective for difficult primary neurons. Micro-structure and collagen coating are made in California. German coverglass is manufactured by German coverslip manufacturers.120 unit package,15 units/pack x 8 packs, ready to use, pre-coated, pre-sterilized by radiation, user manual included

$268.80

MCI-240-Collagen

MCI microfluid primary cell culture insert, the simplest way to establish robust primary cell culture, specially effective for difficult primary neurons. Micro-structure and collagen coating are made in California. German coverglass is manufactured by German coverslip manufacturers.240 unit package,15 units/pack x 16 packs, ready to use, pre-coated, pre-sterilized by radiation, user manual included

$418.80

MCI-60-CUS

MCI microfluid primary cell culture insert, the simplest way to establish robust primary cell culture, specially effective for difficult primary neurons. Micro-structure and custom coating are made in California. German coverglass is manufactured by German coverslip manufacturers. 60 unit package,15 units/pack x 4 packs, pre-coated, pre-sterilized by radiation, ready to use, user manual included

quote

MCI-120-CUS

MCI microfluid primary cell culture insert, the simplest way to establish robust primary cell culture, specially effective for difficult primary neurons. Micro-structure and custom coating are made in California. German coverglass is manufactured by German coverslip manufacturers.120 unit package,15 units/pack x 8 packs, ready to use, pre-coated, pre-sterilized by radiation, user manual included

quote

MCI-240-CUS

MCI microfluid primary cell culture insert, the simplest way to establish robust primary cell culture, specially effective for difficult primary neurons. Micro-structure and custom coating are made in California. German coverglass is manufactured by German coverslip manufacturers.240 unit package,15 units/pack x 16 packs, ready to use, pre-coated, pre-sterilized by radiation, user manual included

quote

 

More readings:

Breakthrough of cell transfection in primary cell culture

Single cell primary neuronal culture

Cortical primary neuron culture

Hippocampal primary neuron culture

Hypothalamic primary neuron culture

Coated coverslips for culturing primary human cells

 

 

Biokeystone

The Bio-Coating Expert

Coated coverslips 

for primary cell cultures

 

Rectangular German glass coverslips

Primary cells culture tested, starting $12/box

 

Round German glass coverslips

Primary cells culture tested, starting $36/box

 

Pre-treated German glass coverslips

Advanced treatment of German glass coverslips, sterile, ready to coat your own substance, starting $78/box

 

Poly-L-lysine coated coverslips

Professional bio-coating technology, primary cell culture tested, sterile, ready to use for cell and neuron cultures, starting $89.50/box

 

Poly-D-lysine coated coverslips

Professional bio-coating technology, primary cell culture tested, sterile, ready to use for cell and neuron cultures, starting $99.50/box

 

Collagen coated coverslips

Professional bio-coating with rat tail type I collagen, primary cell culture tested, sterile, ready to use for cell and neuron cultures, starting $109.50/box

 

Poly-D/L-lysine coated coverslips

Professional bio-coating technology, primary cell culture tested, sterile, ready to use for cell and neuron cultures, starting $99.50/box

 

 

Gelatin coated coverslips

 

 

Custom coated coverslips

 

 

One-step Genotyping DNA extraction kit 

No spin no purification

 

 

  Liberty 120 high speed gel

Features:

Little buffer or buffer-less, 

High-speed electrophoresis in 6 minutes,

Agarose saving,

Straight banding technology,

One tray for 3 gel lengths,  48, 80, or 128mm

 

 

The Problems of primary cell culture:

Serious problems occur in most traditional cell culture systems. For instance, conditioned culture medium is essential to many cell types for optimal growth and responsiveness, but typical large volumes of culture medium dilute the concentrations of secreted cell factors and thus significantly slow down and de-potentiate the life events of the cells in culture. In contrast with in vivo environments, free bulk flow of culture liquid perturbs local chemical gradients, which may impair signaling and disturb cell behaviors. Resulting assay data may be weak, excessively variable and potentially misleading.

 

The Solution:

Using micro-fluidic design and engineering strategies, Biokeystone has developed MCI, a powerful system for cell cultures and co-cultures. With MCI, the free flow of culture medium can be substantially eliminated, stabilizing local gradient profiles of factors secreted by cultured cells. At the same time, the volume of immobilized culture medium surrounding the cultured cells is reduced several hundred fold, which enables rapid accumulation of secreted factors to high levels, accelerating cell establishment, growth, differentiation, or maturation accordingly. If needed, cells can be cultured successfully at very low density. This innovative cell culturing system more nearly mimics in vivo conditions for effective cell signaling and responsiveness, unlike what one sees in traditional cell culture systems.