The professional polylysine coating protocol

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polylysine coating protocol

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Polylysine Coating Protocol

advanced coating techniques   

   

As the leading manufacturer and supplier of poly lysine coated German coverslips, neuVitro has developed a 5-day procedure with our state of the art facility to generate the best professional coating on German coverslips for challenged cell cultures. All coated coverslips are sterilized by radiation and ready to use immediately.

 

Professional poly lysine coating procedure:

Wash coverslips in warm detergent solution x 2 hours.

Rinse coverslips 6 times x 30 minutes each.

Treat coverslips in 100% Ethanol x 2 hour.

Treat coverslips in 70% concentrated Nitric Acid x 24 hours.

Rinse coverslips in dd-water 8 times x 1 hour each.

Coat coverslips with poly-L-lysine or poly-d-lysine, 1mg/ml, Sigma Aldrich:

First layer coating, 1mg/ml

Dry coverslips x 6 hours.

Second layer coating.

Dry coverslips x 6 hours.

Third layer coating.

Dry coverslips x 6 hours.

Rinse coverslips in dd-water 3 times.

Dry coverslips overnight in hood.

Seal coated coverslips into bags, 15 units/bag

Sterilization by radiation

Quality inspection

 

Questions and Answers:

 

How much poly lysine needed to coat my coverslips?

 

The required amount of poly lysine is 7.5ug/cm² surface area of your coverslip. For example, to coat 100 pieces of 18mm diameter coverslips, the total surface area of 100 pieces of coverslips is 0.9x0.9x3.1416 x2x100 = 510cm². The required poly lysine is 510x7.5ug = 3825ug = 3.825mg.

Should I coat coverslips in dd-water or buffer?

 

Poly lysine coating in dd-water is simple. But the pH of dd-water is acidic, usually lower than pH6, which is not optimized for poly lysine coating. Higher pH value, such as pH 8.5 in borate buffer, enhances poly lysine coating. But the buffer residue must be removed thoroughly from coverslips before cell culture.

 

The Bio-Coating Expert

Coating coverslip 

for primary cell culture

 

Rectangular German glass coverslip

Primary cell culture tested, starting $12/box

 

Round German glass coverslip

Primary cell culture tested, starting $36/box

 

Pre-treated German glass coverslip

Advanced treatment of German glass coverslip, sterile, ready to coat your own substance, starting $78/box

 

Poly-L-lysine coated coverslip

Professional bio-coating techniques, primary cell culture tested, sterile, ready to use for cell tissue cultures, starting $89.50/box

 

Poly-D-lysine coated coverslip

Professional bio-coating techniques, primary cell culture tested, sterile, ready to use for cell tissue cultures, starting $99.50/box

 

Collagen coated coverslip

Professional bio-coating with rat tail type I collagen, primary culture tested, sterile, ready to use for tissue culture, starting $109.50/box

 

 

  Cell culture techniques

 

Generating robust accelerated neuron cultures easily. Especially powerful for primary cell culture. Poly-D-lysine coated, optically clear, sterile, ready to use... 

 

  Liberty 120 high speed gel

Features:

Little buffer or buffer-less, 

High-speed electrophoresis in 6 minutes,

Agarose saving,

Straight banding technology,

One tray for 3 gel lengths,  48, 80, or 128mm

 

One-step Genotyping DNA extraction kit 

No spin no purification

 

Fibronectin coating coverslip

 

Gelatin coated coverslip