DNA PCR Method

We did one thing in 16 years and made DNA PCR gel the best

Liberty 120, the most powerful high speed  gel system to see DNA PCR products

PCR technique is now one of the most popular DNA assay method. In PCR analysis, DNA, in trace quantity, functions as template to be amplified. At high PCR temperature, the original DNA is first denatured into single strain structure, then, primers, short pieces of oligos, can bind to the single strain DNA templates specifically according to their match of sequence. DNA polymerase, such as Taqman DNA polymerase, amplifies DNA template via about 30 repeats of a polymerase chain reaction (PCR). In final step of most PCR protocols, the amplified DNA products is visualized by DNA gel electrophoresis (the original DNA template is usually invisible due to its low quantity). By gel electrophoresis, PCR products can be separated from PCR primer due to their size difference. PCR primer runs faster to the front.

Problem of old DNA PCR gel electrophoresis:

Agarose has been widely used for DNA PCR gel electrophoresis in DNA RNA analysis procedures because agarose powder can be easily  heated to form an agarose gel with sample wells. In PCR gel electrophoresis method, for example, a microwave of 1 minute heating can melt agarose powder into buffer solution. The agarose gel solution is then cooled down to form an agarose gel.

The agarose gel formation is readily reversible by heat. Over heating will melt the agarose gel. That is the reason why gel electrophoresis of PCR samples in most labs were performed slowly at low voltage.

The breakthrough of high speed PCR gel electrophoresis in 6 minutes:

By designs of reducing heat generation and cooling enhancement, Biokeystone overcomes the problem of PCR gel melting in electrophoresis. High power drives DNA samples run through PCR gel in just 6 minutes without over heating the agarose gel.

1% DNA PCR gel, 250V 6 minutes run in 1x TAE buffer. PCR band 

For more DNA PCR product of gel electrophoresis, please visit the website and email to info@6mgel.com for user manuals.

Biokeystone