Rapid
PCR electrophoresis in 6 minutes,
See
PCR results in only 6 minutes
US
patent 5,549,806
|
Seen Genotyping PCR
in 6 minutes
Q:
Why 6Mgel PCR gel has the best performance ?
A:
A simple replacement of running buffer by dd water in a
fast PCR screening
system is the trick.
Liberty 120
straight banding technology , 1 gel tray for 3 gel lengths as
shown below
6Mgel
Principle
1x
running buffer, a
highly conductive liquid, has two functions in
conventional submarine agarose gel electrophoresis: keeping the agarose gel cooled
and conducting electric current.
To
avoid gel overheating, the volume of running buffer should be as
large as possible. But more buffer conducts more electric current, which generates more
heat. So buffer volume should be as small as
possible. Which is correct - more buffer or less buffer?
The
principle of the 6Mgel invention
The
inventor of 6mgel recognized that water is the best and cheapest
coolant. The electrical resistance of lab dd-water is 18MΩ,
which means that dd-water is an excellent electrical
insulator. By simply adding dd-water directly on top of the
buffer-containing agarose gel, the desired effects are achieved:
gels
can be run much faster! You can add a large volume of
dd-water to dissipate heat so that a much higher voltage can be
applied to drive faster migration of DNA molecules. Band "smiling" is eliminated because
the large volume of dd-water in direct contact with the gel
maintains even heat distribution. Also, sharper banding occurs
due to "stacking effects of dd-water in gel wells.
In
6Mgel, the V/cm in wells is elevated about 3-fold higher than in
the gel body, which drives DNA 3 times faster in wells than in
the gel body, resulting in sharper bands. Furthermore,
consumption of running buffer can be reduced by 50-75%.
The
strength of 6Mgel is particularly significant for Genotyping and PCR
applications, where high percentage agarose gels are usually required.
It takes longer time for sample molecules to migrate through the
concentrated gel body. 6Mgel innovation has solved the problem.
During
the development of 6Mgel, more and more features were
added, such as buffer-less high speed electrophoresis, lab-made
precast gels for high-speed electrophoresis, and agarose-saving
gel systems. Go to 6mgel systems for
more information.
Please
feel free to ask 6Mgel if you have further questions. Thanks you!
