Rapid
Neuron
Culture
Generating accelerated neuron
culture easily
|
US and
international patents pending
MCI
-- Microfluid Primary Neuron Culture
Generating
robust neuron culture easily. Especially powerful for
primary neuron culture. Poly-L-lysine or poly-D-lysine coated, optically
clear, sterilized by radiation, ready to use. MCI
is designed for challenged primary neuron cultures. Significant improvement can be
expected if you have experienced difficulties of culturing primary
neurons.
The handling
of MCI culture system is very simple:
It
keeps your working habit of using multi-well plates for
cell cultures. Each unit of MCI can be placed into, and
detached from, wells of
multi-well plates.
You will still
perform
desired procedures the same way as usual, such as viewing and
imaging live neurons, changing culture medium, performing neuron transfections,
trypsinizing and
harvesting cells, fixing neurons and IHC staining,
or mounting cultured neurons on slides after staining.
|
Rapid Neuron
culture in Microfluid neuronal culture, click to see enlarged neuron |
MCI:
Neuron culture Insert, US and international patents
pending, Micro-structure and PLL coating made in USA, sterile, ready for
primary neuron cultures
|
List
of coated coverslips for neuron culture
| Cat.
# |
Poly-L-lysine
coated coverslips |
Price |
| GG-12-PLL |
12mm
diameter, # 1 thickness, 90
pieces of Poly L lysine coated coverslips, CE certified coverglass, sterilized by
radiation, sealed with15 pieces per bag, ready to use for neuron
culture, fitting to 24-well cell culture plate or cell
culture dish |
$89.50 |
| GG-12-PLL-180 |
12mm
diameter, # 1 thickness, 180
pieces of Poly L lysine coated coverslips, CE certified coverglass, sterilized by
radiation, sealed with15 pieces per bag, ready to use for neuron culture, fitting to 24-well cell culture plate or cell
culture dish |
$159.00 |
| GG-14-PLL |
14mm
diameter, # 1 thickness, 90
pieces of Poly L lysine coated coverslips, CE certified coverglass, sterilized by
radiation, sealed with15 pieces per bag, ready to use for neuron culture, fitting to 12-well cell culture plate or cell
culture dish |
$89.50 |
| GG-14-PLL-180 |
14mm
diameter, # 1 thickness, 180
pieces of Poly L lysine coated coverslips, CE certified coverglass, sterilized by
radiation, sealed with15 pieces per bag, ready to use for neuron culture, fitting to 12-well cell culture plate or cell
culture dish |
$159.00 |
| GG-15-PLL |
15mm
diameter, # 1 thickness, 90
pieces of Poly L lysine coated coverslips, CE certified coverglass, sterilized by
radiation, sealed with15 pieces per bag, ready to use
neuron culture, fitting to 12-well cell culture plate or cell
culture dish |
$89.50 |
| GG-15-PLL-180 |
15mm
diameter, # 1 thickness, 180
pieces of Poly L lysine coated coverslips, CE certified coverglass, sterilized by
radiation, sealed with15 pieces per bag, ready to use
neuron culture, fitting to 12-well cell culture plate or cell
culture dish |
$159.00 |
| GG-18-PLL |
18mm
diameter, # 1 thickness, 90
pieces of Poly L lysine coated coverslips, CE certified coverglass, sterilized by
radiation, sealed with15 pieces per bag, ready to use for
neuron culture, fitting to 12-well cell culture plate or
cell culture dish |
$92.50 |
| GG-18-PLL-180 |
18mm
diameter, # 1 thickness, 180
pieces of Poly L lysine coated coverslips, CE certified coverglass, sterilized by
radiation, sealed with15 pieces per bag, ready to use for
neuron culture, fitting to 12-well cell culture plate or
cell culture dish |
$165.00 |
| GG-20-PLL |
20mm
diameter, # 1 thickness, 90
pieces of Poly L lysine coated coverslips, CE certified coverglass, sterilized by
radiation, sealed with15 pieces per bag, ready to use for neuron culture, fitting to 12-well cell culture plate or cell
culture dish |
$92.50 |
| GG-20-PLL-180 |
20mm
diameter, # 1 thickness, 180
pieces of Poly L lysine coated coverslips, CE certified coverglass, sterilized by
radiation, sealed with15 pieces per bag, ready to use for neuron culture, fitting to 12-well cell culture plate or cell
culture dish |
$165.00 |
| GG-22-PLL |
22mm
diameter, # 1 thickness, 90
pieces of Poly L lysine coated coverslips, CE certified coverglass, sterilized by
radiation, sealed with15 pieces per bag, ready to use for
neuron culture, fitting to 6-well cell culture plate or
cell culture dish |
$92.50 |
| GG-22-PLL-180 |
22mm
diameter, # 1 thickness, 180
pieces of Poly L lysine coated coverslips, CE certified coverglass, sterilized by
radiation, sealed with15 pieces per bag, ready to use for
neuron culture, fitting to 6-well cell culture plate or
cell culture dish |
$165.00 |
| GG-24-PLL |
24mm
diameter, # 1 thickness, 90
pieces of Poly L lysine coated coverslips, CE certified coverglass, sterilized by
radiation, sealed with15 pieces per bag, ready to use for neuron culture, fitting to 6-well cell culture plate or cell
culture dish |
$95.50 |
| GG-24-PLL-180 |
24mm
diameter, # 1 thickness, 180
pieces of Poly L lysine coated coverslips, CE certified coverglass, sterilized by
radiation, sealed with15 pieces per bag, ready to use for neuron culture, fitting to 6-well cell culture plate or cell
culture dish |
$169.00 |
| GG-25-PLL |
25mm
diameter, # 1 thickness, 90
pieces of Poly L lysine coated coverslips, CE certified coverglass, sterilized by
radiation, sealed with15 pieces per bag, ready to use for neuron culture, fitting to 6-well cell culture plate or cell
culture dish |
$99.50 |
| GG-25-PLL-180 |
25mm
diameter, # 1 thickness, 90
pieces of Poly L lysine coated coverslips, CE certified coverglass, sterilized by
radiation, sealed with15 pieces per bag, ready to use for neuron culture, fitting to 6-well cell culture plate or cell
culture dish |
$179.00 |
| GG-25-0-PLL |
25mm
diameter, # 0 thickness, 60
pieces of Poly L lysine coated coverslips, CE certified coverglass, sterilized by
radiation, sealed with15 pieces per bag, ready to use for neuron culture, fitting to 6-well cell culture plate or cell
culture dish |
$105.50 |
| GG-25-0-PLL-120 |
25mm
diameter, # 0 thickness, 120
pieces of Poly L lysine coated coverslips, CE certified coverglass, sterilized by
radiation, sealed with15 pieces per bag, ready to use for neuron culture, fitting to 6-well cell culture plate or cell
culture dish |
$179.00 |
| GG-25-1.5-PLL |
25mm
diameter, # 1.5 thickness, 90
pieces of Poly L lysine coated coverslips, CE certified coverglass, sterilized by
radiation, sealed with15 pieces per bag, ready to use for neuron culture, fitting to 6-well cell culture plate or cell
culture dish |
$105.50 |
| GG-25-1.5-PLL-180 |
25mm
diameter, # 1.5 thickness,180
pieces of Poly L lysine coated coverslips, CE certified coverglass, sterilized by
radiation, sealed with15 pieces per bag, ready to use for neuron culture, fitting to 6-well cell culture plate or cell
culture dish |
$179.00 |
Also
see uncoated German coverslips starting from $12/box
| Cat.
# |
Poly-D-lysine
coated coverslips |
Price |
| GG-12-pdl |
12mm
diameter, # 1 thickness, 90
pieces of Poly D lysine coated coverslips, CE certified German coverglass, sterilized by
radiation, sealed with15 pieces per bag, ready to use for
neuron culture, fitting to 24-well cell culture plate
or cell culture dish |
$99.50 |
| GG-12-pdl-180 |
12mm
diameter, # 1 thickness, 180
pieces of Poly D lysine coated coverslips, CE certified German coverglass, sterilized by
radiation, sealed with15 pieces per bag, ready to use for
neuron culture, fitting to 24-well cell culture plate
or cell culture dish |
$179.00 |
| GG-14-pdl |
14mm
diameter, # 1 thickness, 90
pieces of Poly D lysine coated coverslips, CE certified German coverglass, sterilized by
radiation, sealed with15 pieces per bag, ready to use for
neuron culture, fitting to 12-well cell culture plate
or cell culture dish |
$99.50 |
| GG-14-pdl-180 |
14mm
diameter, # 1 thickness, 180
pieces of Poly D lysine coated coverslips, CE certified German coverglass, sterilized by
radiation, sealed with15 pieces per bag, ready to use for
neuron culture, fitting to 12-well cell culture plate
or cell culture dish |
$179.00 |
| GG-15-pdl |
15mm
diameter, # 1 thickness, 90
pieces of Poly D lysine coated coverslips, CE certified German coverglass, sterilized by
radiation, sealed with15 pieces per bag, ready to use for neuron culture, fitting to 12-well cell culture plate or
cell culture dish |
$99.50 |
| GG-15-pdl-180 |
15mm
diameter, # 1 thickness, 180
pieces of Poly D lysine coated coverslips, CE certified German coverglass, sterilized by
radiation, sealed with15 pieces per bag, ready to use for neuron culture, fitting to 12-well cell culture plate or
cell culture dish |
$179.00 |
| GG-18-pdl |
18mm
diameter, # 1 thickness, 90
pieces of Poly D lysine coated coverslips, CE certified German coverglass, sterilized by
radiation, sealed with15 pieces per bag, ready to use for neuron culture, fitting to 12-well cell culture plate or
cell culture dish |
$102.50 |
| GG-18-pdl-180 |
18mm
diameter, # 1 thickness, 180
pieces of Poly D lysine coated coverslips, CE certified German coverglass, sterilized by
radiation, sealed with15 pieces per bag, ready to use for neuron culture, fitting to 12-well cell culture plate or
cell culture dish |
$189.00 |
| GG-20-pdl |
20mm
diameter, # 1 thickness, 90
pieces of Poly D lysine coated coverslips, CE certified German coverglass, sterilized by
radiation, sealed with15 pieces per bag, ready to use for neuron culture, fitting to 12-well cell culture plate or
cell culture dish |
$102.50 |
| GG-20-pdl-180 |
20mm
diameter, # 1 thickness, 180
pieces of Poly D lysine coated coverslips, CE certified German coverglass, sterilized by
radiation, sealed with15 pieces per bag, ready to use for neuron culture, fitting to 12-well cell culture plate or
cell culture dish |
$189.00 |
| GG-22-pdl |
22mm
diameter, # 1 thickness, 90
pieces of Poly D lysine coated coverslips, CE certified German coverglass, sterilized by
radiation, sealed with15 pieces per bag, ready to use for neuron culture, fitting to 6-well cell culture plate or cell
culture dish |
$102.50 |
| GG-22-pdl-180 |
22mm
diameter, # 1 thickness, 180
pieces of Poly D lysine coated coverslips, CE certified German coverglass, sterilized by
radiation, sealed with15 pieces per bag, ready to use for neuron culture, fitting to 6-well cell culture plate or cell
culture dish |
$189.00 |
| GG-24-pdl |
24mm
diameter, # 1 thickness, 90
pieces of Poly D lysine coated coverslips, CE certified German coverglass, sterilized by
radiation, sealed with15 pieces per bag, ready to use for neuron culture, fitting to 6-well cell culture plate or cell
culture dish |
$105.50 |
| GG-24-pdl-180 |
24mm
diameter, # 1 thickness, 180
pieces of Poly D lysine coated coverslips, CE certified German coverglass, sterilized by
radiation, sealed with15 pieces per bag, ready to use for neuron culture, fitting to 6-well cell culture plate or cell
culture dish |
$195.00 |
| GG-25-pdl |
25mm
diameter, # 1 thickness, 90
pieces of Poly D lysine coated coverslips, CE certified German coverglass, sterilized by
radiation, sealed with10 pieces per bag, ready to use for neuron culture, fitting to 6-well cell culture plate or cell
culture dish |
$105.50 |
| GG-25-pdl-180 |
25mm
diameter, # 1 thickness, 180
pieces of Poly D lysine coated coverslips, CE certified German coverglass, sterilized by
radiation, sealed with10 pieces per bag, ready to use for neuron culture, fitting to 6-well cell culture plate or cell
culture dish |
$195.00 |
| GG-25-0-pdl |
25mm
diameter, # 0 thickness, 60
pieces of Poly D lysine coated coverslips, CE certified German coverglass, sterilized by
radiation, sealed with10 pieces per bag, ready to use for neuron culture, fitting to 6-well cell culture plate or cell
culture dish |
$115.50 |
| GG-25-0-pdl-120 |
25mm
diameter, # 0 thickness, 120
pieces of Poly D lysine coated coverslips, CE certified German coverglass, sterilized by
radiation, sealed with10 pieces per bag, ready to use for neuron culture, fitting to 6-well cell culture plate or cell
culture dish |
$195.00 |
| GG-25-1.5-pdl |
25mm
diameter, # 1.5 thickness, 90
pieces of Poly D lysine coated coverslips, CE certified German coverglass, sterilized by
radiation, sealed with10 pieces per bag, ready to use for neuron culture, fitting to 6-well cell culture plate or
cell culture dish |
$115.50 |
| GG-25-1.5-pdl-180 |
25mm
diameter, # 1.5 thickness, 180
pieces of Poly D lysine coated coverslips, CE certified German coverglass, sterilized by
radiation, sealed with10 pieces per bag, ready to use for neuron culture, fitting to 6-well cell culture plate or
cell culture dish |
$209.00 |
Products
of MCI Microfluid Neuron Culture Insert
|
Cat
#
|
Product
Description
|
Unit
price
|
|
MCI-60-PLL
|
MCI
microfluid neuron culture insert, the simplest way to establish robust
neuronal culture, specially effective for difficult primary
neurons. Micro-structure and poly-L-lysine coating are made in
California. German coverglass is manufactured by German
coverslip manufacturers. 60
unit package,15 units/pack x 4 packs, pre-coated,
pre-sterilized by radiation, ready to use, user manual included
|
$148.80
|
|
MCI-120-PLL
|
MCI
microfluid neuron culture insert, the simplest way to establish robust
neuronal culture, specially effective for difficult primary
neurons. Micro-structure and poly-L-lysine coating are made in
California. German coverglass is manufactured by German
coverslip manufacturers.120
unit package,15 units/pack x 8 packs, ready to use, pre-coated,
pre-sterilized by radiation, user manual included
|
$248.80
|
|
MCI-240-PLL
|
MCI
microfluid neuron culture insert, the simplest way to establish robust
neuronal culture, specially effective for difficult primary
neurons. Micro-structure and poly-L-lysine coating are made in
California. German coverglass is manufactured by German
coverslip manufacturers.240
unit package,15 units/pack x 16 packs, ready to use, pre-coated,
pre-sterilized by radiation, user manual included
|
$398.80
|
|
MCI-60-PDL
|
MCI
microfluid neuron culture insert, the simplest way to establish robust
neuronal culture, specially effective for difficult primary
neurons. Micro-structure and poly-D-lysine coating are made in
California. German coverglass is manufactured by German
coverslip manufacturers. 60
unit package,15 units/pack x 4 packs, pre-coated,
pre-sterilized by radiation, ready to use, user manual included
|
$148.80
|
|
MCI-120-PDL
|
MCI
microfluid neuron culture insert, the simplest way to establish robust
neuronal culture, specially effective for difficult primary
neurons. Micro-structure and poly-D-lysine coating are made in
California. German coverglass is manufactured by German
coverslip manufacturers.120
unit package,15 units/pack x 8 packs, ready to use, pre-coated,
pre-sterilized by radiation, user manual included
|
$248.80
|
|
MCI-240-PDL
|
MCI
microfluid neuron culture insert, the simplest way to establish robust
neuronal culture, specially effective for difficult primary
neurons. Micro-structure and poly-D-lysine coating are made in
California. German coverglass is manufactured by German
coverslip manufacturers.240
unit package,15 units/pack x 16 packs, ready to use, pre-coated,
pre-sterilized by radiation, user manual included
|
$398.80
|
|
MCI-60-Collagen
|
MCI
microfluid neuron culture insert, the simplest way to establish robust
neuronal culture, specially effective for difficult primary
neurons. Micro-structure and collagen coating are made in
California. German coverglass is manufactured by German
coverslip manufacturers. 60
unit package,15 units/pack x 4 packs, pre-coated,
pre-sterilized by radiation, ready to use, user manual included
|
$168.80
|
|
MCI-120-Collagen
|
MCI
microfluid neuron culture insert, the simplest way to establish robust
neuronal culture, specially effective for difficult primary
neurons. Micro-structure and collagen coating are made in
California. German coverglass is manufactured by German
coverslip manufacturers.120
unit package,15 units/pack x 8 packs, ready to use, pre-coated,
pre-sterilized by radiation, user manual included
|
$268.80
|
|
MCI-240-Collagen
|
MCI
microfluid neuron culture insert, the simplest way to establish robust
neuronal culture, specially effective for difficult primary
neurons. Micro-structure and collagen coating are made in
California. German coverglass is manufactured by German
coverslip manufacturers.240
unit package,15 units/pack x 16 packs, ready to use, pre-coated,
pre-sterilized by radiation, user manual included
|
$418.80
|
|
MCI-60-CUS
|
MCI
microfluid neuron culture insert, the simplest way to establish robust
neuronal culture, specially effective for difficult primary
neurons. Micro-structure and custom coating are made in
California. German coverglass is manufactured by German
coverslip manufacturers. 60
unit package,15 units/pack x 4 packs, pre-coated,
pre-sterilized by radiation, ready to use, user manual included
|
quote
|
|
MCI-120-CUS
|
MCI
microfluid neuron culture insert, the simplest way to establish robust
neuronal culture, specially effective for difficult primary
neurons. Micro-structure and custom coating are made in
California. German coverglass is manufactured by German
coverslip manufacturers.120
unit package,15 units/pack x 8 packs, ready to use, pre-coated,
pre-sterilized by radiation, user manual included
|
quote
|
|
MCI-240-CUS
|
MCI
microfluid neuron culture insert, the simplest way to establish robust
neuronal culture, specially effective for difficult primary
neurons. Micro-structure and custom coating are made in
California. German coverglass is manufactured by German
coverslip manufacturers.240
unit package,15 units/pack x 16 packs, ready to use, pre-coated,
pre-sterilized by radiation, user manual included
|
quote
|
|
|
Biokeystone
The
Bio-Coating Expert
Coated
coverslips
for
neuron cultures
|
 |
Primary cell
and neuron
culture tested, starting $12/box
Primary cell
and neuron
culture tested, starting $36/box
Advanced
treatment of German glass coverslips, sterile, ready to coat your own
substance, starting $78/box
Professional
bio-coating technology, primary cell
culture tested, sterile, ready to use for cell and neuron cultures,
starting $89.50/box
Professional
bio-coating technology, primary cell
culture tested, sterile, ready to use for cell and neuron cultures,
starting $99.50/box
Professional
bio-coating with rat tail type I collagen, primary cell
culture tested, sterile, ready to use for cell and neuron cultures,
starting $109.50/box
Professional
bio-coating technology, primary cell
culture tested, sterile, ready to use for cell and neuron cultures,
starting $99.50/box
No
spin no purification
 Liberty
120 high speed gel
Features:
Little
buffer or buffer-less,
High-speed
electrophoresis in 6 minutes,
Agarose
saving,
Straight
banding technology,
One
tray for 3 gel
lengths, 48, 80, or 128mm
How
MCI works?
The
Problems of old neuron culture:
Serious problems occur in most traditional
neuron culture
systems. For instance, conditioned culture medium is essential for optimal growth and
responsiveness of cultured neurons, but typical large
volumes of culture medium dilute the concentrations of secreted factors and thus significantly slow down and de-potentiate the life
events of the neurons in culture. In contrast with in vivo environments,
free bulk flow of culture liquid perturbs local chemical gradients,
which may impair signaling and disturb neuron behaviors. Resulting assay
data may be weak, excessively variable and potentially misleading.
The
Solution:
Using
micro-fluidic design and engineering strategies, Biokeystone has developed
MCI, a powerful system for neuron culture and co-culture. With MCI, the free flow of
culture medium can be substantially eliminated, stabilizing local
gradient profiles of factors secreted by cultured cells. At the same
time, the volume of immobilized culture medium surrounding the cultured
cells is reduced several hundred fold, which enables rapid accumulation
of secreted factors to high levels, accelerating cell establishment,
growth, differentiation, or maturation accordingly. If needed, cells can
be cultured successfully at very low density. This innovative cell
culturing system more nearly mimics in vivo conditions for effective cell
signaling and responsiveness, unlike what one sees in traditional
culture systems.
--------------------------------------------------
Think you
already have the best transfection kit for cells in tissue culture?
Think again! See how to make huge differences by a simple thinking...
--------------------------------------------------
Tips of
neuron culture:
Tip
#1:
Pay close attention to consistency of your
neuronal culture
Do
you know that your effort of culturing neurons in vitro will gain
NOTHING useful if you do not pay particular attention to maintain
consistency in your operation. You should start with a very detailed
protocol and follow the protocol without changing parameters, including
the counts of your dissociated neuron cells, the platting area on cover
glasses, the amount of Neurobasal medium, the schedule of culture medium
change, the time length and frequency of observation of your neurons in
culture, etc.
Tip
#2:
Use P1 instead of E18 for primary cortical
neuron culture
culturing
neurons using E18 tissue is a labor-intensive problematical neuronal
culture protocol, including checking plugs, lack of genotyping
information, difficulties in precise tissue dissection, and the most
importantly, unnecessary loss of animal life. Biokeystone is the expert of
using P1 tissue for all primary neuronal cultures, including protocols
of cortical culture, hippocampal culture, hypothalamus culture,
astrocyte culture, and motor neuron culture.
Tip
#3:
Do not shake your incubator while culturing
neurons
In
primary neuronal culture, we create a manmade situation with the hope
that neuron cells can adapt and survive in artificial culture medium,
then regain their life as normal. But did you know that a shaking of
culture incubator can stop the growth of cultured neurons for a pretty
long period of time? Irregular interruption can generate significant
variation in your neuron culture data. To overcome this problem,
Biokeystone is developing special systems for culturing primary neurons for
more stable and consistent outcome. Back
|
