Microfluid Neuronal Culture System

Generating robust accelerated cultures easily

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MCI -- Microfluid Neuronal Culture Insert

MCI is designed for primary neuronal cultures and co-cultures. It generates robust accelerated neuron cultures. Each unit of MCI is PLL, PDL, or collagen coated, optically clear, sterile, ready to use...

The handling of the MCI:

It keeps your working habit of using multi-well plates for cell cultures. Each unit of MCI can be placed into, and removed from, wells of multi-well plates.

You will still perform desired procedures the same way as usual, such as viewing and imaging live neurons, changing culture medium, performing neuron transfections, trypsinizing and harvesting cells, fixing neurons and IHC, or mounting cultured neurons on slides after staining.


Energized and accelerated culture of primary neurons Pure low density primary neuron cultured in defined simple culture medium using MCI neuronal culture system	MCI: Neuron Microfluid Culture Insert, US and international patents pending, Micro structure and PLL coated, sterile, ready for culture.

US and international patents pending

MCI products

Cat #	Product Description	Price	Order online
MCI-60	60 units MCI microfuid cell culture insert, the simplest way to establish robust primary cultures, specially effective for difficult primary cell types. Micro bio-coating and fabrications are made in California. German cover glasses are imported from German coverslip manufacturers.	$148.80
MCI-120	120 units MCI microfuid cell culture insert, the simplest way to establish robust primary cultures, specially effective for difficult primary cell types. Micro bio-coating and fabrications are made in California. German cover glasses are imported from German coverslip manufacturers.	$248.80
MCI-240	240 units MCI microfuid cell culture insert, the simplest way to establish robust primary cultures, specially effective for difficult primary cell types. Micro bio-coating and fabrications are made in California. German cover glasses are imported from German coverslip manufacturers.	$398.80

Neuronal Culture Products

MCI Principle:

The problems of old cell culture systems:

Serious problems occur in most traditional cell culture systems. For instance, conditioned culture medium is essential to many cell types for optimal growth and responsiveness, but typical large volumes of culture medium dilute the concentrations of secreted cell factors and thus significantly slow down and de-potentiate the life events of the cells in culture. In contrast with in vivo environments, free bulk flow of culture liquid perturbs local chemical gradients, which may impair signaling and disturb cell behaviors. Resulting assay data may be weak, excessively variable and potentially misleading.

The Solution:

Using micro-fluidic design and engineering strategies, Biokeystone has developed MCI, a powerful system for cell cultures and co-cultures. With MCI, the free flow of culture medium can be substantially eliminated, stabilizing local gradient profiles of factors secreted by cultured cells. At the same time, the volume of immobilized culture medium surrounding the cultured cells is reduced several hundred fold, which enables rapid accumulation of secreted factors to high levels, accelerating cell establishment, growth, differentiation, or maturation accordingly. If needed, cells can be cultured successfully at very low density. This innovative cell culturing system more nearly mimics in vivo conditions for effective cell signaling and responsiveness, unlike what one sees in traditional culture systems.

Biokeystone - The Bio-Coating Expert

Coated coverslips for neuronal cultures

Rectangular German glass coverslips

Primary neuron culture tested, starting $12/box

Round German glass coverslips

Primary neuron culture tested, starting $36/box

Pre-treated German glass coverslips

Advanced treatment of German glass coverslips, sterile, ready to coat your own substance, starting $78/box

Poly-L-lysine coated coverslips

Professional bio-coating technology, primary neuronal culture tested, sterile, ready to use for neuron cultures, starting $89.50/box

Poly-D-lysine coated coverslips

Professional bio-coating technology, primary neuronal culture tested, sterile, ready to use for cell and neuron cultures, starting $99.50/box

Collagen coated coverslips

Professional bio-coating with rat tail type I collagen, primary cell culture tested, sterile, ready to use for cell and neuron cultures, starting $109.50/box

Poly-D/L-lysine coated coverslips

Professional bio-coating technology, primary cell culture tested, sterile, ready to use for neuron cultures, starting $99.50/box

the cell culture expert