Gel Electrophoresis, Protocols, Tips, tools, and theories

Gel electrophoresis is a daily operation in many labs. Have you thought about how good is you gel electrophoresis? Is the protocol of traditional gel electrophoresis you are using a good one? Think again now, you will see how much you can change for your daily gel electrophoresis experiments.

Smart Tips of gel electrophoresis protocols and tips:

How to screening 96 PCR samples in 6 minute?

How to change gel length between 2cm to 12cm for agarose saving?

How to pour your own precast gels for fast gel electrophoresis?

How to eliminate gel smiling?

How to generate even ethidium bromide staining?

Systems for novel gel electrophoresis:

Febe gel electrophoresis system with features of casting 20 gels at once and use them for a month.

Midear gel electrophoresis system with features of length change from 2cm to 12cm.

Super 120 gel electrophoresis system,

Liberty 1 gel electrophoresis system,

RAGE gel electrophoresis system,

TAE gel electrophoresis buffer:

3 Liters of 50X TAE for gel electrophoresis

2 Liters of 50X TAE for gel electrophoresis

1 Liters of 50X TAE for gel electrophoresis 

3 Liters of 10X TAE for gel electrophoresis 

2 Liters of 10X TAE for gel electrophoresis 

1 Liters of 10X TAE for gel electrophoresis 

TBE gel electrophoresis buffer:

3 Liters of 10X TBE for gel electrophoresis

2 Liters of 10X TBE for gel electrophoresis

1 Liters of 10X TBE for gel electrophoresis 

3 Liters of 5X TBE for gel electrophoresis 

2 Liters of 5X TBE for gel electrophoresis 

1 Liters of 5X TBE for gel electrophoresis 

Gel Electrophoresis Theory

Gel electrophoresis is a technique used to separate molecules based on their size and charge, according to the following equation where v = the rate (velocity) of migration, E is the strength of the electrical field, z is the charge on the molecule and f is the frictional force on the molecule
                                                V=EZ / F
where v = the rate (velocity) of migration, E is the strength of the electrical field, z is the charge on the molecule and f is the frictional force on the molecule. 

In zonal gel electrophoresis, cations in solution migrate toward either the cathode of gel electrophoresis (negatively charged) whereas anions migrate toward the anode of gel electrophoresis (positively charged) when an electrical field is applied. The principles of zonal electrophoresis are used mainly in capillary electrophoresis these days.

In gel electrophoresis, a matrix consisting of either polyacrylamide (for proteins and small nucleic acids) or agarose (for larger nucleic acids) is prepared. A polyacrylamide gel contains long linear polymers of acrylamide that are cross-linked to each other using bis-acrylamide. Samples are applied in sample wells at the cathodic end of the gel matrix. When an electric field is applied, negatively charged species migrate toward the anode.

In gel electrophoresis the gel serves two purposes. It serves to diffuse convective currents that would result in localized heating in the matrix, which would result in irregular migration patterns. The gel also creates a molecular sieve that enhances the separation based on molecular weight. 

Gel Electrophoresis related term Definitions:

Agarose 

A large pore polymer used as a gel medium in electrophoresis of large molecules, particularly nucleic acids

Amino acids 

The building blocks of proteins. The twenty alpha-amino acids are used to synthesize proteins in all living systems. Some of these amino acids have charged side chains which can contribute to the charge behavior of proteins during electrophoresis.

Anode 

The positively charged electrode that attracts negatively charged proteins

Buffer 

A weak acid or base that resists change in pH

Denaturation 

A process by which the non-covalent bonds (hydrogen bonds, electrostatic forces, van der Waals forces) of proteins are broken thereby unfolding the protein into a random coil. SDS is used in electrophoresis to denature proteins.

DTT 

Dithiothreitol is a reducing agent used to break the disulfide bonds
between cysteine residues in polypeptides.

Capillary Electrophoresis 

A technique where an electrical field is applied to either end of a capillary (a small diameter silicon tube). Molecules in the capillary then migrate based on their size and charge.

Cathode 

The negatively charged node that attracts positively charged proteins

Convection currents 

Naturally produced currents due to the movement of warm air. These currents affect the electrophoresis because the sample is constantly moving and therefore it cannot be guaranteed that all the polypeptide chains are completely separated from each other.

Electrophoresis 

A process by which proteins are separated based on their mobility in a gel medium

Glycoproteins 

Proteins with a covalently bonded carbohydrate attached

Lipoproteins Proteins bonded to a lipid

Mercaptoethanol 

A reducing agent

Molecular weight 

The sum of the atomic weights in a molecule which is expressed in amus (atomic mass units). The molecular weight of a molecule is directly related to its migration distance in an electrophoresis gel, the smaller the molecular weight the farther the polypeptide migrates, and the larger the molecular weight the less the polypeptide migrates

Native 

The native conformation is the original conformation of a protein molecule

Polyacrylamide 

Used as a gel medium in electrophoresis because it acts as a friction force on the migrating polypeptide chains

Primary structure 

A straight chain sequence of amino acids

Protein 

Constructed from polypeptides (sequences of 20 amino acids) which are folded/coiled into unique conformations

Random coil 

A random or irregularly bent/folded section of a polypeptide chain with a secondary structure. This irregularity provides a means for the polypeptide chain to bend back on itself.

Quaternary structure 

A globular protein created from two or more polypeptide chains bonded together

Reducing agents 

Substances such as DTT and mercaptoethanol which reduce a protein to its primary structure.

Relative migration Rm 

The amount a polypeptide chain moves in relation to the tracking dye. Also called the relative mobility, it is calculated by dividing the distance the polypeptide migrates by the distance the tracking dye migrates.

SDS 

Sodium dodecyl sulfate

Secondary structure 

Polypeptide chains with folds/coils due to hydrogen bonding between CO and NH groups

Sodium dodecyl sulfate 
A detergent used to denature proteins thereby unfolding the protein to its primary structure

Subunits 

Polypeptides which make up the quarternary structure of a protein

Tertiary structure 

A polypeptide chain with folds/coils as well as bonding between side chains creating a 3D shape as a result of hydrophobic interactions and disulfide bridges