 |
 |
Biokeystone Solving-gel-smiling-problem |
Get 6mgel to eliminate gel smiling Generate your data faster easier and better |
|
|
Home About us Contact Us Technologies Products Ordering Order Status Warranty |
||||
Click "Order Status" to see who are using Biokeystone's smiling-free gel system
The main causes of gel smiling problem:a. Uneven distribution of electric field across gel width b. Uneven elevation of temperature among different lanes As a user, you can do nothing about uneven electric field (your gel system has been permanently manufactured that way). The only thing you can do is to run the gel slowly so that the gel temperature unevenness is less significant.
6mgel, Smiling-free high speed gel system in 6 minutesThe innovative feature of straight banding technology: a. Edge compensation of electrode for even electric field b. Direct dd-water cooling on gel top for uniform temperature distribution
Using 6Mgel, the problem of gel smiling is simply eliminated, as shown below. The Breakthrough of high-speed electrophoresis without running bufferSee User Feedback about the performance of buffer-less high speed electrophoresis:
6Mgel Principle of smiling free technologies
1x running buffer, a highly conductive liquid, has two functions in conventional submarine agarose gel electrophoresis: keeping the agarose gel cooled and conducting electric current.
To avoid gel overheating, the volume of running buffer should be as large as possible. But more buffer conducts more electric current, which generates more heat. So buffer volume should be as small as possible. Which is correct - more buffer or less buffer?
The principle of the 6Mgel inventionThe inventor of 6mgel recognized that water is the best and cheapest coolant. The electrical resistance of lab dd-water is 18MΩ, which means that dd-water is an excellent electrical insulator. By simply adding dd-water directly on top of the buffer-containing agarose gel, the desired effects are achieved: gels can be run much faster! You can add a large volume of dd-water to dissipate heat so that a much higher voltage can be applied to drive faster migration of DNA molecules. Band "smiling" is eliminated because the large volume of dd-water in direct contact with the gel maintains even heat distribution. Also, sharper banding occurs due to "stacking effects of dd-water in gel wells. In 6Mgel, the V/cm in wells is elevated about 3-fold higher than in the gel body, which drives DNA 3 times faster in wells than in the gel body, resulting in sharper bands. Furthermore, consumption of running buffer can be reduced by 50-75%.
The strength of 6Mgel is particularly significant for Genotyping and PCR applications, where high percentage agarose gels are usually required. It takes longer time for sample molecules to migrate through the concentrated gel body. 6Mgel innovation has solved the problem.
During the development of 6Mgel, more and more features were added, such as buffer-less high speed electrophoresis, lab-made precast gels for high-speed electrophoresis, and agarose-saving gel systems. Go to 6mgel systems for more information. |
|
