6Mgel
instrument
Run
gel in dd-water with straight banding
|
Click "Order
Status" to see who are using 6mgel technologies.
Electrophoresis instrument
and tips for smarter gel electrophoresis:
The
Principle of 6Mgel rapid electrophoresis in only 6 minutes
1x
running buffer, a
highly conductive liquid, has two functions in
conventional submarine agarose gel electrophoresis: keeping the agarose gel cooled
and conducting electric current.
To
avoid gel overheating, the volume of running buffer should be as
large as possible. But more buffer conducts more electric current, which generates more
heat. So buffer volume should be as small as
possible. Which is correct - more buffer or less buffer?
Breakthrough of high speed
electrophoresis with no running buffer
See User Feedback about the performance of buffer-less high speed electrophoresis:
" The
Liberty
bufferless gel system is the best gel electrophoresis system we've ever
used. It's not only easy to set up, robust, and fast, but generates
excellent results without the need of running buffer. Highly
recommended. See our genotyping service picture below." ---- Ivan Delgado Orlic, President and CEO,
Mouse
Genotype Co.
|
The
principle of the 6Mgel invention
The
inventor of 6mgel recognized that water is the best and cheapest
coolant. The electrical resistance of lab dd-water is 18MΩ,
which means that dd-water is an excellent electrical
insulator. By simply adding dd-water directly on top of the
buffer-containing agarose gel, the desired effects are achieved:
gels can be run much faster! You can add a large volume of
dd-water to dissipate heat so that a much higher voltage can be
applied to drive faster migration of DNA molecules. Gel
smiling is eliminated because the large volume of dd-water
in direct contact with the gel maintains even heat distribution.
Also, sharper banding occurs due to "stacking effects of
dd-water in gel wells.
In
6Mgel, the V/cm in wells is elevated about 3-fold higher than in
the gel body, which drives DNA 3 times faster in wells than in
the gel body, resulting in sharper bands. Furthermore,
consumption of running buffer can be reduced by 50-75%.
During
the development of 6Mgel, more and more features were
added, such as buffer-less high speed electrophoresis, lab-made
precast gels for high-speed electrophoresis, and agarose-saving
gel systems. Go to 6mgel instruments for
more information.
Liberty
120 gel instrument operation steps:
1.
Select gel length, 48, 88, or 128mm to meet sample quantity and running
distance requirement.
2.
Pour agarose gel solution into gel tray, place combs into comb slots,
and let the agarose gel to set.
3.
Remove combs, add 350ml of dd-water on gel top and 200ml of 1x running
buffer into buffer reservoir.
4.
Load samples into sample wells. Well distance is multi-channel pipette
compatible.
5.
Run rapid gel electrophoresis at 220V DC for 6-10 minutes depending on
your applications.
6.
Take gel pictures on UV transilluminator.
Liberty
120 gel instrument quality features:
UV
transparent bottom is built with specially enforced UV trasmitting
acrylic for working under UV light without cause cracking or aging. Gel
chamber is built with heavy gauge solid blocks of Plexiglass
Q: Why Biokeystone gel
electrophoresis instrument is irresistible ?
A: It is clear if you
know the feature of Biokeystone horizontal gel electrophoresis instrument ,
Since 1994, Biokeystone invention of submarine gel electrophoresis instrument
has passed over 10 model versions. The name of the latest model tells how
Biokeystone gel electrophoresis instrument are differ from any other gel
electrophoresis instrument.
Febe
gel electrophoresis instrument
Faster
Easier Bufferless Electrophoresis
Run high-speed electrophoresis in
6-minute with no running buffer.
Cast
20 gels at once and store them for a month without any wrapping.
Have
your own precast gels ready anytime without paying high prices of
commercial gels.
Straight
banding technology eliminates gel-smiling problem.
Super gel
electrophoresis, including super 120 and super 80.
Screener gel
electrophoresis, including super 13.
Scooter gel
electrophoresis, including scooter 100, scooter 60.
Liberty gel
electrophoresis, including liberty 1, liberty 2, liberty 4.
Q gel electrophoresis
RAGE gel
electrophoresis, including RGX100 RGX 60.
Why Biokeystone gel electrophoresis
instrument is the best?
In agarose gel electrophoresis instrument, the instrument
chamber holds liquid buffer, such as TAE buffer, TBE buffer, and mops buffer. An
agarose gel plate is immersed under the conductive electrophoresis running
buffer. The electrophoresis buffer conducts electric current two electrodes of
the electrophoresis equipments. Sample molecules in the electric field migrate
along a direction from negative to positive according to their charge and
molecular size. After agarose gel electrophoresis process, the gel plate is
removed from the electrophoresis equipment to a UV illuminator for
picturing. Sample molecules form distinguished bands in the agarose gel
plate.
The conductive electrophoresis buffer in the electrophoresis
instrument, while conducting electric current to agarose gel plate, generates
undesired heat during agarose gel electrophoresis, which limits agarose gel
electrophoresis a slow process at low voltage.
Biokeystone invents water cooling agarose gel electrophoresis
instrument, a unique agarose gel electrophoresis equipment with so many
advantages together, and leads agarose gel electrophoresis industry to modern
generation.
High speed gel straight banding in 6 minutes
How
to run precast gel electrophoresis instrument using no running buffer?
How
to screening 96 PCR samples in 6 minute with Febe electrophoresis instrument?
Agarose
gel electrophoresis instruments
How
to run electrophoresis using no running buffer?
How
to run electrophoresis application on 96 PCR samples in 6 minute?
