Electrophoresis applications: Tips and protocols

For electrophoresis applications including DNA application, RNA applications, protein applications, etc. For those electrophoresis applications, two main kinds of electrophoresis systems are utilized, horizontal gel system and vertical gel system.

In horizontal gel system for electrophoresis applications of DNA RNA analysis, an agarose gel is immersed under running buffer. Samples are applied into sample wells. Then an electric field is applied to the agarose gel for causing electrophoresis of sample molecules.

In vertical gel system for electrophoresis applications of protein analysis, a polyacrylamide gel is formed between a pair of glasses. Protein samples run through the gel and form distinguishable bands after electrophoresis according to their molecule sizes.

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