EDTA stock solution for rapid gel electrophoresis

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EDTA stock solution recipe

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Use dd-Water to save EDTA stock solution

 

 

EDTA 0.5M Stock solution

EDTA= Ethylenediamine tetra acetic acid

 Na2 EDTA 2 H2O, MW 372.24, is the chemical for preparing 0.5M EDTA stock.

Chemicals Final concentration Volume Weight
Na2 EDTA 2 H2O 0.5 M   93.05 g
H2O   Start with 300ml  

 

  1. Stir vigorously on magnetic stirrer. You will need about 10 g NaOH pellets. This step takes some time.

  2. Add NaOH pellets gradually to avoid over dose of NaOH. The EDTA powder does not go into solution before enough NaOH pellets being added. 

  3. Adjust volume to 480 ml with ddH2O. 

  4. Adjust ph to 8.0, 

  5. Bring the final volume to 500ml. 

The EDTA solution is stable for long-term storage at room temperature.

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