DNA
gel electrophoresis
High-speed
agarose gel in 6 minutes
|
DNA gel electrophoresis
DNA is one of
the most common sample for agarose gel electrophoresis, such as genotyping, PCR,
plasmid DNA,
and genomic DNA southern blotting gel. During DNA gel electrophoresis, DNA molecules run through agarose gel under electric field
during gel electrophoresis process. The speed of DNA migration in gel electrophoresis depends on voltage and
temperature. Uneven electric field and temperature distribution will cause gel smiling
in DNA gel electrophoresis.
Smart Tips of DNA gel
electrophoresis:
How
to run DNA gel electrophoresis using only 15ml running buffer?
How
to run 96 PCR samples in DNA gel electrophoresis for only 6 minute?
The basics of agarose gel for DNA gel
electrophoresis
In agarose DNA gel electrophoresis, an agarose gel plate is
immersed under conductive electrophoresis running buffer, such as TAE buffer,
TBE buffer, and mops buffer. The DNA gel electrophoresis buffer conducts electric
current from electrodes to the agarose gel plate. DNA sample molecules in the
electric field migrate, in a direction from negative to positive, according to
DNA charge and DNA molecular size. After agarose DNA gel electrophoresis process,
DNA sample molecules form distinguished bands in the agarose gel plate.
The conductive electrophoresis buffer, while conducting
electric current to agarose gel plate, generates undesired heat during agarose
DNA gel electrophoresis, which limits agarose DNA gel electrophoresis a slow process at
low voltage
Biokeystone invents Febe agarose DNA gel electrophoresis system, a
unique agarose gel equipment with so many advantages together, and leads agarose
DNA
gel electrophoresis industry to modern generation
DNA gel electrophoresis in 6 minutes
