DNA gel electrophoresis 

DNA is one of  the most common sample for agarose gel electrophoresis, such as genotyping, PCR, plasmide DNA, and genomic DNA southern blotting gel. During DNA gel electrophoresis, DNA molecules run through agarose gel under electric field during gel electrophoresis process. The speed of DNA migration in gel electrophoresis depends on voltage and temperature. Uneven electric field and temperature distribution will cause gel smiling in DNA gel electrophoresis. 

Smart Tips of DNA gel electrophoresis: 

How to run DNA gel electrophoresis using only 15ml running buffer?

How to run 96 PCR samples in DNA gel electrophoresis for only 6 minute?

How to change gel length between 2cm to 12cm for agarose saving in DNA gel electrophoresis?

How to save up to 90% antibodies in immunohistochemistry?

The basics of agarose gel for DNA gel electrophoresis

In agarose DNA gel electrophoresis, an agarose gel plate is immersed under conductive electrophoresis running buffer, such as TAE buffer, TBE buffer, and mops buffer. The DNA gel electrophoresis buffer conducts electric current from electrodes to the agarose gel plate. DNA sample molecules in the electric field migrate, in a direction from negative to positive, according to DNA charge and DNA molecular size. After agarose DNA gel electrophoresis process, DNA sample molecules form distinguished bands in the agarose gel plate.

The conductive electrophoresis buffer, while conducting electric current to agarose gel plate, generates undesired heat during agarose DNA gel electrophoresis, which limits agarose DNA gel electrophoresis a slow process at low voltage.

Biokeystone invents Febe and Midear agarose  DNA gel electrophoresis system, a unique agarose gel equipment with so many advantages together, and leads agarose  DNA gel electrophoresis industry to modern generation.

DNA gel electrophoresis in 6 minutes