DNA
electrophoresis
Rapid
gel in 6 minutes
|
DNA Electrophoresis
DNA is one of
the most common sample for electrophoresis, such as genotyping, PCR, plasmide,
and genomic DNA southern blotting gel. During DNA
electrophoresis, DNA molecules run through agarose gel under electric field
during electrophoresis process. The speed of DNA migration in electrophoresis depends on voltage and
temperature. Uneven electric field and temperature distribution will cause gel smiling
in DNA electrophoresis.
Smart Tips of DNA electrophoresis:
How
to run DNA electrophoresis using no running buffer?
How
to run 96 PCR samples in DNA electrophoresis for only 6 minute?
See User Feedback about the performance of buffer-less high speed electrophoresis:
" The
Liberty
bufferless gel system is the best gel electrophoresis system we've ever
used. It's not only easy to set up, robust, and fast, but generates
excellent results without the need of running buffer. Highly
recommended. See our genotyping service picture below." ---- Ivan Delgado Orlic, President and CEO,
Mouse
Genotype Co.
|
The basics of agarose gel for DNA electrophoresis
In agarose DNA electrophoresis, an agarose gel plate is
immersed under conductive electrophoresis running buffer, such as TAE buffer,
TBE buffer, and mops buffer. The DNA electrophoresis buffer conducts electric
current from electrodes to the agarose gel plate. DNA sample molecules in the
electric field migrate, in a direction from negative to positive, according to
DNA charge and DNA molecular size. After agarose DNA gel electrophoresis process,
DNA sample molecules form distinguished bands in the agarose gel plate.
The conductive electrophoresis buffer, while conducting
electric current to agarose gel plate, generates undesired heat during agarose
gel electrophoresis, which limits agarose gel DNA electrophoresis a slow process at
low voltage.
Biokeystone invents many agarose gel DNA electrophoresis
systems with so many advantages together and leads agarose
gel DNA electrophoresis industry to advanced generation.
