MCI:
The
Best Co-Culture System
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MCI
-- Microfluid Co-Culture Insert
Generating
robust cell cultures and co-cultures easily. Especially powerful for
primary cell cultures and co-cultures. PLL/collagen coated, optically
clear,
sterile, ready to use...
Product
format:
The handling
of the MCI is easy and convenient:
It
keeps your working habit of using multi-well plates for
cell cultures. Each unit of MCI can be placed into, and
detached from, wells of
multi-well plates.
You will still
perform
desired procedures the same way as usual, such as viewing and
imaging live neurons, changing culture medium, performing neuron transfections,
trypsinizing and
harvesting cells, fixing neurons and IHC,
or mounting cultured neurons on slides after staining.
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Pure live primary neurons in
culture viewed under phase contrast microscope. Pure
primary neuron cultured in defined simple culture medium.
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MCI:
Neuron Microfluid Culture Insert, US and international patents
pending, Micro structure and PLL coated, sterile, ready for
culture. |
US and
international patents pending
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Cat
#
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Product
Description
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Unit
price
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MCI-60-C
|
MCI
micro cell co-culture system, the simplest way to establish robust primary
co-
cultures, specially effective
for difficult primary cell types. Micro bio-coating
and fabrications are made in California. German cover
glasses are imported from German coverslip manufacturers.
60 unit
package,15 units/pack x 4 packs, ready to use, pre-coated,
pre-sterilized.
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$148.80
|
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MCI-120-C
|
MCI
micro cell co-culture system, the simplest way to establish robust primary
co-
cultures, specially effective
for difficult primary cell types. Micro bio-coating
and fabrications are made in California. German cover
glasses are imported from German coverslip manufacturers.
120 unit
package, 15 units/pack x 8 packs
|
$248.80
|
|
MCI-240-C
|
MCI
micro cell co-culture system, the simplest way to establish robust primary
co-
cultures, specially effective
for difficult primary cell types. Micro bio-coating
and fabrications are made in California. German cover
glasses are imported from German coverslip manufacturers.
240 unit
package, 15 units/pack x 16 packs
|
$398.80
|
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The
best Co-Culture System
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How
can our Microfluid Co-Culture Insert make your cell co-cultures robust?
The Problem:
Serious problems occur in most traditional cell co-culture
systems. For instance, conditioned culture medium is essential to many
cell types for optimal growth and responsiveness, but typical large
volumes of culture medium dilute the concentrations of secreted cell
factors and thus significantly slow down and de-potentiate the life
events of the cells in co-culture. In contrast with in vivo environments,
free bulk flow of culture liquid perturbs local chemical gradients,
which may impair signaling and disturb cell behaviors. Resulting assay
data may be weak, excessively variable and potentially misleading.
The
Solution:
Using
micro-fluidic design and engineering strategies, Biokeystone has developed
MCI, a new model for cell co-culture technology. With MCI, the free flow of
culture medium can be substantially eliminated, stabilizing local
gradient profiles of factors secreted by co-cultured cells. At the same
time, the volume of immobilized culture medium surrounding the co-cultured
cells is reduced several hundred fold, which enables rapid accumulation
of secreted factors to high levels, accelerating cell establishment,
growth, differentiation, or maturation accordingly. If needed, cells can
be co-cultured successfully at very low density. This innovative co-culture
system more nearly mimics in vivo conditions for effective cell
signaling and responsiveness, unlike what one sees in traditional co-culture
systems.
Applications of
co-cultures:
Co-culture is
a very useful experimental approach to study interactions between two
different kinds of cells in vitro, such as coculture of neurons and
glial cells, coculture of stem cells and feed layers, coculture of
NIH/3T3 fibrobalsts and monocyte /macrophages, coculture of embryo and
endometrial or tubal cells, coculture of bone marrow fibrobasts and
endothelial cells, coculture of mammary fibroblasts and MCF-7 cells,
coculture of yeast and bacterium, coculture of epithelial cells and
bacteria.....
The two kinds
of different cells must be very closed to each other during co-culture
period. But they should be easily separated from each other after the
co-culture. MCI co-culture system has a minimized micro co-culture space
in micro-meter range, which enhances the interactions of the two cell
types in co-culture dramatically.
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