Agarose gel loading dye

Tips to improve agarose gel loading dye in agarose gel electrophoresis

Agarose gel loading dye has following functions:

1. Make your sample visible after mixing with agarose gel loading dye.

2. Make your sample heaver than running buffer.

3. Trap your sample within the wells of your agarose gel.

But from time to time, agarose gel loading dye cause you trouble. For example, your band will be invisible when it runs at the same location as your agarose gel loading dye colors. Your sample will run slower if the salt condition is not optimized in your sample.

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