50x TAE buffer, Tris-acetate-EDTA buffer, 

50X TAE Buffer pH 7.6-7.8

To make 3L:
726g Tris
300mL of 0.5M EDTA pH 8.0
171.3mL Glacial Acetic Acid
Add enough MQ H2O to dissolve solids, pH with HCl to 7.6-7.8, then bring up to final volume of 3000mL

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