50X TAE Buffer pH 7.6-7.8

To make 2L:
484g Tris
200mL of 0.5M EDTA pH 8.0
114.2mL Glacial Acetic Acid
Add enough MQ H2O to dissolve solids, pH with HCl to 7.6-7.8, then bring up to final volume of 2000mL

 

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                                  50x tae buffer 2 liter

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