High speed gel running in little or no TAE buffer

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 Rapid-TAE--buffer-gel-system

Run high speed gel and save TAE buffer

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Run gel in dd-Water

US Patent 5,549,806

 

Click Order Status to see who are using dd-water high-speed gel systems.

 

TAE (Tris-Acetate-EDTA) buffer has advantages over TBE buffer. Unlike TBE, you can prepare TAE buffer as 50x concentrated stock solution without worrying about precipitation.

 

Recipes and tips for 1 liter of standard 50X TAE Buffer:

242 g Trizma Base + 100ml 0.5M EDTA pH 8.0 +57.2ml Glacial acetic acid

Simplified method to make 1 liter of the standard 50x TAE buffer:

Use a 1000ml bottle, add about 600ml ddwater and put a stir bar into the bottle to mix 242g Trizma base +100ml of 0.5M EDTA pH 8.0 + 57.2ml glacial acetic acid, then bring final volume to 1000ml with additional ddwater. There is no need to adjust pH of the final 50x TAE stock solution.

Prepared 50x TAE stock solution can be stored at room temperature for a long period of time. Please note, Trizma base is a brand name of Tris-base.

Tips:

How to prepare 0.5M EDTA stock solution pH 8.0 ?

Among the three components of 50x TAE buffer, EDTA should be prepared as 0.5M stock solution with pH adjustment to 8.0, which takes some time and skill. For your convenience, here is how to prepare 0.5M EDTA stock solution.

How to increase sample running speed using your old gel chamber?

 

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The problems of your TAE gel system:

 

 

Diagram above shows traditional submarine gel electrophoresis. 1x running buffer, a highly conductive liquid, has two functions: keeping the agarose gel cooled and conducting electric current.

 

To avoid gel overheating and band smiling, the volume of running buffer should be as large as possible. But more buffer conducts more electric current, which generates more heat. So buffer volume should be as small as possible. Which is correct - more buffer or less buffer?

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