1.5 agarose gel, the magic 6-minute electrophoresis system for PCR screening

Febe system uses 1.5 agarose gel to run 200V high speed gel electrophoresis in 6 minutes. 1.5 agarose gel is the most common concentration for agarose gel electrophoresis. 1.5 agarose gel can be used for high speed electrophoresis to separate DNA RNA molecules from 100bp to 2kb. All brands of agarose powder can be used to form 1.5 agarose gel for the purpose. Biokeystone patent technology allows 1.5 agarose gel electrophoresis at high voltage over 200V. Even cooling water keep 1.5 agarose gel in uniform temperature across all sample lanes

Below are list of receipt for making 1.5 agarose gel in different volume

For 150ml of 1.5 agarose gel solution, dissolve 2.25 g agarose powder 

For 125ml of 1.5 agarose gel solution, dissolve 1.875 g agarose powder 

For 100ml of 1.5 agarose gel solution, dissolve 1.5 g agarose powder 

For 80ml of 1.5 agarose gel solution, dissolve 1.2 g agarose powder 

For 60ml of 1.5 agarose gel solution, dissolve 0.9 g agarose powder 

For 50ml of 1.5 agarose gel solution, dissolve 0.75 g agarose powder 

For 40ml of 1.5 agarose gel solution, dissolve 0.6 g agarose powder 

For 30ml of 1.5 agarose gel solution, dissolve 0.45 g agarose powder 

For 20ml of 1.5 agarose gel solution, dissolve 0.3 g agarose powder 

 

More tips:

How to run precast gel using only 15ml running buffer?

How to screening 96 PCR samples in 6 minute?

How to change gel length between 2cm to 12cm for agarose saving? 

 

                                  1.5 agarose

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