High Speed gel without TBE running buffer

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Rapid-TBE--buffer-gel-system

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Run gel in dd-Water

US Patent 5,549,806

 

Click Order Status to see who are using dd-water high-speed gel systems.

 

Recipes for 1 liter of standard 10X TBE Buffer:

108g Trizma base + 55g Boric acid + 40mls 0.5M EDTA (pH 8.0)

Simplified method to make 1 liter of the standard 10x TBE buffer:

Use a 1000ml bottle, add about 600ml ddwater and put a stir bar into the bottle to mix 108g Trizma base + 55g Boric acid + 40mls 0.5M EDTA (pH 8.0), then bring final volume to 1000ml with additional ddwater. There is no need to adjust pH of the final 10x TBE buffer stock solution.

Prepared 10x TBE buffer stock solution can be stored at room temperature for a short period of time. Precipitation will form at bottom of storage bottle. Precipitated 10x TBE buffer stock should be disposed. Please note, Trizma base is a brand name of Tris-base.

Tips:

How to prepare stock solution of 0.5M EDTA ph8.0 ?

Among the three components of 10x TBE buffer, EDTA should be prepared as 0.5M stock solution with pH adjustment to 8.0, which takes some time and skill. For your convenience, here is further reading for preparation of 0.5M EDTA stock solution.

 

How to increase sample running speed using your old gel chamber?

 

Patent technology: Rapid 6-minute Electrophoresis in dd-Water

 

Straight banding as shown below

" The Liberty bufferless gel system in dd-water is the best gel electrophoresis system we've ever used. It's not only easy to set up, robust, and fast, but generates excellent results without the need of running buffer. Highly recommended." ---- Ivan Delgado Orlic, President and CEO, Mouse Genotype Co. 

Genotyping gel of Mouse Genotype Co using Liberty gel system running high-speed in dd-water.

 

 

 

Cell Culture Coverslips

 

Rectangular German glass coverslips

Primary cell and neuron culture tested, starting $12/box

 

Poly-L-lysine coated coverslips

Primary cell culture tested, sterile, ready to use for cell and neuron cultures, starting $89.50/box

 

Collagen coated coverslips

Primary cell culture tested, sterile, ready to use for cell and neuron cultures, starting $109.50/box

 

Round German glass coverslips

Primary cell and neuron culture tested, starting $36/box

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The problems of your TBE gel system:

 

 

Diagram above shows traditional submarine gel electrophoresis. 1x running buffer, a highly conductive liquid, has two functions: keeping the agarose gel cooled and conducting electric current.

 

To avoid gel overheating and band smiling, the volume of running buffer should be as large as possible. But more buffer conducts more electric current, which generates more heat. So buffer volume should be as small as possible. Which is correct - more buffer or less buffer?

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