10x TAE buffer

To make 3L:
145.2g Tris
60mL of 0.5M EDTA pH 8.0
34.26mL Glacial Acetic Acid
Add enough MQ H2O to dissolve solids, pH with HCl to 7.6-7.8, then bring up to final volume of 3000mL

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                                  10x tae buffer 3 liter

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