6Mgel Tips:
Use
dd-Water to save TAE buffer
|
How to prepare standard 10X TAE Buffer &
save your TAE buffer?
To prepare 2 Liter of 10x TAE buffer, use a
2000ml bottle with 1500ml of deionized water and add:
96.8g Trizma base
40mL of 0.5M EDTA pH 8.0
22.84mL Glacial Acetic Acid, then bring final volume to2000ml.
See User Feedback about the performance of buffer-less high speed electrophoresis:
" The
Liberty
bufferless gel system is the best gel electrophoresis system we've ever
used. It's not only easy to set up, robust, and fast, but generates
excellent results without the need of running buffer. Highly
recommended. See our genotyping service picture below." ---- Ivan Delgado Orlic, President and CEO,
Mouse
Genotype Co.
|
Biokeystone has modified standard TAE buffer so that you can
have your own modified TAE buffer with much better performance.
Q:
Why 6M gel
electrophoresis apparatus has the best performance ?
A:
A simple change of TAE buffer with water as shown in diagram
below
In 6mgel setting,
electrodes contact agarose gel directly so that TAE running buffer is
eliminated. Cooling water absorbs heat from agarose gel evenly and quickly,
which enables high-speed electrophoresis and obtains straight banding.
