Run
gel in dd-Water
US
Patent 5,549,806
|
Click Order
Status to see who are using dd-water high-speed gel systems.
TAE (Tris-Acetate-EDTA)
buffer has
advantages over TBE buffer. Unlike TBE, you can prepare TAE buffer as
10x
concentrated stock solution without worrying about precipitation.
Recipes for
1 liter of standard 10X TAE
Buffer:
48.4g Trizma base + 20ml of 0.5M EDTA pH 8.0 + 11.44ml
glacial acetic acid
Simplified method to make 1 liter of
the standard 10x TAE buffer:
Use a 1000ml bottle,
add about 600ml ddwater and put a stir bar into the bottle to mix
48.4g Trizma base +20ml of 0.5M EDTA pH 8.0 + 11.44ml
glacial acetic acid, then
bring final volume to 1000ml with additional ddwater. There is no need
to adjust pH of the final 10x TAE buffer stock solution.
Prepared 10x TAE
buffer stock
solution can be stored at room temperature for a long period of time. Please
note,
Trizma base is a brand name of Tris-base.
Tips:
Among
the three components of 10x TAE buffer, EDTA should be prepared as 0.5M
stock solution with pH adjustment to 8.0, which takes some time and
skill. For your convenience, here is further
reading for preparation of 0.5M EDTA stock solution.
Patent
technology: Rapid
6-minute Electrophoresis in dd-Water
Straight
banding as shown below
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" The Liberty bufferless gel
system in dd-water is the best gel electrophoresis
system we've ever used. It's not only easy to set up,
robust, and fast, but generates excellent results
without the need of running buffer. Highly
recommended." ---- Ivan Delgado Orlic, President
and CEO, Mouse Genotype Co. |
Genotyping
gel of Mouse Genotype Co using Liberty gel system running
high-speed in dd-water.
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---------------------------------------
The
problems of your TAE gel system:
Diagram
above shows traditional submarine gel electrophoresis. 1x
running buffer, a highly conductive liquid, has two functions:
keeping the agarose gel cooled and conducting electric current.
To
avoid gel overheating and band smiling, the volume of running
buffer should be as large as possible. But more buffer conducts
more electric current, which generates more heat. So buffer
volume should be as small as possible. Which
is correct - more buffer or less buffer?
Read
more
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