1. Increase the
density of the sample, ensuring that the DNA drops evenly into the well to
form straight bands, and to eliminate protein interference.
2. Add color to
the sample, thereby simplifying the loading process;
3. Contain dyes
that, in an electric field, move toward the anode at predictable rates.
Bromophenol blue migrates through agarose gels approximately 2.2-fold faster
than xylene cyanol FF, independent of the agarose concentration. Bromophenol
blue migrates through agarose gels run in 0.5× TBE at approximately the
same rate as linear double-stranded DNA 300 bp in length, whereas xylene
cyanol FF migrates at approximately the same rate as linear double-stranded
DNA 4kb in length. These relationships are not significantly affected by the
concentration of agarose in the gel over the range of 0.5% to 1.4%.
See User Feedback about the performance of buffer-less high speed electrophoresis:
" The
Liberty
bufferless gel system is the best gel electrophoresis system we've ever
used. It's not only easy to set up, robust, and fast, but generates
excellent results without the need of running buffer. Highly
recommended. See our genotyping service picture below." ---- Ivan Delgado Orlic, President and CEO,
Mouse
Genotype Co.
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