10X agarose gel loading buffer

Biokey gel-loading buffers are made with molecular grade high purity chemicals. No DNase RNase detectable.

DNA gel loading buffer serves three purposes:

	1. Increase the density of the sample, ensuring that the DNA drops evenly into the well to form straight bands, and to eliminate protein interference.
	2. Add color to the sample, thereby simplifying the loading process;
	3. Contain dyes that, in an electric field, move toward the anode at predictable rates. Bromophenol blue migrates through agarose gels approximately 2.2-fold faster than xylene cyanol FF, independent of the agarose concentration. Bromophenol blue migrates through agarose gels run in 0.5× TBE at approximately the same rate as linear double-stranded DNA 300 bp in length, whereas xylene cyanol FF migrates at approximately the same rate as linear double-stranded DNA 4kb in length. These relationships are not significantly affected by the concentration of agarose in the gel over the range of 0.5% to 1.4%.

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10x agarose gel loading buffer

The irresistible beauty of novel biotechniques from Biokeystone

Cat.#	Description of 10x agarose gel loading buffer	Price	Order online
LB10-DG2	2ml of 10X DNA gel loading buffer made with molecular grade high purity chemical Glycerol	$35
LB10-DG5	5ml of 10X DNA gel loading buffer made with molecular grade high purity chemical Glycerol	$60
LB10-DS2	2ml of 10X DNA gel loading buffer made with molecular grade high purity chemical Sucrose	$35
LB10-DS5	5ml of 10X DNA gel loading buffer made with molecular grade high purity chemical Sucrose	$60
LB10-DF2	2ml of 10X DNA gel loading buffer made with molecular grade high purity chemical Ficoll	$45
LB10-DF5	5ml of 10X DNA gel loading buffer made with molecular grade high purity chemical Ficoll	$80