0.8 agarose, Its properties and recipe

0.8 agarose is useful for agarose gel electrophoresis of DNA RNA molecules longer than 6kb. Concentration higher than 0.8 agarose will result in poor separation.

But 0.8 agarose is very fragile. Care should be taken during electrophoresis of the 0.8 agarose. Avoid the highest voltage electrophoresis which can cause melting of 0.8 agarose gel.

Below are list of receipt for making 0.8 agarose in different volume

For 150ml of 0.8 agarose gel solution, dissolve 1.2 g agarose powder 

For 125ml of 0.8 agarose gel solution, dissolve 1 g agarose powder 

For 100ml of 0.8 agarose gel solution, dissolve 0.8 g agarose powder 

For 80ml of 0.8 agarose gel solution, dissolve 0.64 g agarose powder 

For 60ml of 0.8 agarose gel solution, dissolve 0.48 g agarose powder 

For 50ml of 0.8 agarose gel solution, dissolve 0.4 g agarose powder 

For 40ml of 0.8 agarose gel solution, dissolve 0.32 g agarose powder 

For 30ml of 0.8 agarose gel solution, dissolve 0.24 g agarose powder 

For 20ml of 0.8 agarose gel solution, dissolve 0.16 g agarose powder 

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